Journal Information
Vol. 8. Issue 1.
Pages 752-758 (January - March 2019)
Download PDF
More article options
Vol. 8. Issue 1.
Pages 752-758 (January - March 2019)
Original Article
DOI: 10.1016/j.jmrt.2018.05.018
Open Access
Apatite flotation using saponified baker's yeast cells (Saccharomyces cerevisiae) as a bioreagent
André Carlos Silvaa,
Corresponding author

Corresponding author.
, Diego Valentim Crescente Carab, Elenice Maria Schons Silvac, Geraldo Sadoyama Leald, Alex Malüe Machadoe, Lucas Martins da Silvae
a Modelling and Mineral Processing Research Lab, Engineering Faculty, Federal University of Goiás, CAPES scholarship POS-DOC-88881.119296/2016-01, Catalão, Brazil
b Post-Doctorate Fellowship FAPEG/CNPq, Federal University of Goiás, Catalão, Brazil
c Postgraduate Program in Metallurgical, Materials, and Mining Engineering, Federal University of Minas Gerais, CNPq Scholarship SWE-206196/2017-2, Belo Horizonte, Brazil
d Biological Sciences Department, Biotechnology Faculty, Federal University of Goiás, Catalão, Brazil
e Mineral Processing Post-Graduation Course, Engineering Faculty, Federal University of Goiás, Catalão, Brazil
This item has received

Under a Creative Commons license
Article information
Full Text
Download PDF
Figures (5)
Show moreShow less
Tables (2)
Table 1. XRF results for the apatite samples (in %).
Table 2. Apatite flotation data set using 96h-aged saponified BYC.
Show moreShow less

Brazil has many mineral resources, one of them being phosphate rocks. Established in 1978, the current main mineral-processing route for the Brazilian phosphate rock igneous deposits consists in a direct flotation using alkaline-saponified fatty acids as collector. Looking for new sources of reactants to be used in mineral processing, commercial baker's yeast cells (BYC) from Fleishmann were tested. Saccharomyces cerevisiae was chosen because it is relatively easy to grow industrially, has no biological risk, and can be found worldwide. Microflotation experiments were conducted in a modified Hallimond's tube with high-purity apatite samples in order to investigate the influence of the pH and the biocollector dosage on the apatite recovery. High saponification levels for the BYC occurred many hours after its saponification and an optimal 96h aging was established. The industrially adopted collector (Flotigam 5806 from Clariant) was used as collector benchmark. Apatite recoveries with the 96h-aged saponified BYC were higher than with Flotigam 5806 (above 95% in all tested pH for dosages of saponified BYC ≥200mg/L). Good results were found also at pH 7, which could lead to a reduction in the phosphate rock flotation pH in the future. No major differences were visually found between the 96h-aged saponified BYC and the Flotigam 5806 regarding the froth aspect and stability, suggesting that the biocollector possibly acted as a frother.

Saccharomyces cerevisiae
Hallimond tube
Full Text

Nowadays, Brazil's economy is strongly based on agricultural commodities [1]. Its arable territory and tropical climate put Brazil as one of the world's largest producer of vegetables and meat, and among the world's six largest agricultural producer and exporter [2]. However, most of Brazilian soils present low fertility and does not fit the crop's nutritional requirements. Therefore, chemical fertilizers must be used [3,4]. This places Brazil both as one of the biggest exporters of agricultural products and as one of the biggest importers of fertilizers [5].

The three essential elements for plant nutrition are nitrogen, phosphorus, and potassium. The Brazilian main source of phosphorous is phosphate rock from igneous deposits, which requires mineral processing to reduce the content of gangue minerals, such as quartz, chert, clay, feldspar, mica, calcite, and dolomite [6]. Flotation is the major concentration method [7] using alkaline-saponified fatty acids as collectors [8]. The fatty acids-based collectors can be synthetic or extracted from different vegetable species. Conventional chemicals used in other flotation systems, such as petroleum derivate, xanthates, cyanides, and amines, are toxic, non-degradable, and exorbitantly expensive in nature [9].

The application of microorganisms in mineral processing is not relatively new, but is still in the early stages [10]. It started as a solution to actual problems in mineral flotation, such as the depletion of high-grade ores, the necessity of concentrate fine and ultrafine particles, and to the constant quest of environmental friendly reagents [11]. For the authors, the term bioflotation turned out attractive not only as the solution of the problems mentioned before, but also to its technological potential and mineral selectivity. Bioflotation exploits the differences in surface characteristics of solids suspended in an aqueous medium, adjusting and controlling their surface energies and interfacial tensions using microorganisms with hydrophobic properties [10]. In their opinion, a significant increase in the researches related to the electrophoretic behavior of minerals/bacteria systems, thermodynamic adhesion of different bioreagents onto mineral surface, and bioflotation kinetics, to name a few, are required in order to have a better understanding of the fundamental aspects of mineral bioflotation. Mineral bioflotation encompasses the principles and methods used in mineral flotation using microorganisms as flotation reagents.

The attachment of bacteria on the mineral surface occurs by a variety of methods such as the secretion of a slime layer, hydrophobic interactions, extracellular polymeric substances and other surface appendages, protein-binding receptors, chemical attachment, physical adsorption, and with the use of glycolyax [12]. The feasibility of adherence of a microorganism to a mineral surface will depend on the charge characteristics as well as the hydrophobicity of both mineral surface and microorganism [13]. Microorganisms already identified for their potential application in microbial-induced flotation and flocculation are considerably diverse and include hydrophobic and surfactant producing species of bacteria, fungi, and some species of yeast [14].

Saccharomyces cerevisiae (SC) commonly refereed as baker's yeast cells (BYC) is widely used to produce bread. Lipids, proteins, and two types of polysaccharides (α-mannan and α-glucan) mainly compose it [15]. SC had been used in bioflotation before, but only after submitted to adaptation for the targeted minerals and without being saponified. For example, calcite was successfully separated from quartz using mineral-adapted cells of SC [16]. BYC had potential to be used as a biocollector in mineral flotation mainly not only because of its performance as biocollector, but also because of its price, relatively easy to grow industrially, and high biological safety and availability. In this paper, the effect of the pH, dosage, and aging of the saponified BYC on the apatite recovery was studied. The results show a possible use of saponified BYC as apatite collector.

2Materials and methods2.1Mineral samples

Blue crystals of igneous apatite from Ipira-BA, Brazil, were comminuted in a jaw crusher followed by a ball mill and granulometrically separated through wet sieving using a Tyler sieving series for 15min. The samples were then dried in an oven at 60°C for 24h. A ferrite magnet with a field of 2kG was used to remove any possible contamination from the previous stages. The mineralogical phase characterization was performed using an X-ray diffractometer Rigaku Miniflex II with Cu tube and Cu Kα radiation, graphite monochromator, operating at 30kV/15mA, angular step of 0.02˚, and acquisition time of 2s. The sample chemical composition was determined using an X-ray fluorescence spectrometer PANalytical AXIOX MAX series DY 5001. The sample images were acquired with an SEM JEOL JSM-6610 coupled with EDS probe Thermo Scientific NSS Spectral Imaging. The particle size analyzed was performed wet with the addition of Na2P2O7 (1g/L) as dispersant agent and tap water using a laser diffraction particle size analyzer Sympatec HELOS. The apatite zeta potential was measured at the pH range from 3.5 to 12.5 using distilled water and KCl (10−3mol/L) as indifferent electrolyte using a Zetasizer Malvern Nano ZS90.

2.2BYC preparation and saponification

Dry BYC manufactured by Fleishmann were locally purchased in 100g packages, containing the cells and emulsifying agents. The BYC was solubilized in distilled water to produce a solution of 20mL/g of BYC. The solution was centrifuged at room temperature for 5min at 3400rpm in order to remove the emulsifying agents. The obtained pellets were suspended in distilled water. The centrifugation loop was repeated three times, under the same conditions described. The final pellets were dried in oven at 70°C for 48h. The processed BYC were disaggregated using a stainless steel mortar and pestle.

A solution of 100g of processed BYC and 600mL of distilled water (pH 7) was added into a 1L glass beaker under magnetic stirring at 130rpm at room temperature until there was complete solubilization of the solids. The BYC saponification was performed by the addition of 400g of NaOH solution (10%, w/v) to the solution.

The saponification index (SI), which is the mass of potassium hydroxide (in mg) required to neutralize the fatty acids resulting from the complete hydrolysis of 1g of fat, was used to study the BYC saponification reaction kinetics. Previous results indicated that BYC saponification kinetics was slow, requiring the aging of the solution before its use as a collector. To test the hypothesis that BYC requires some aging to achieve high apatite recoveries, saponified BYC solutions were prepared and their SI was measured. The methodology adopted for the SI measurement was adapted from [17], where 100mL of saponified BYC was stirred with 25mL of methanolic KOH (1N) and the excess of the mixture was then titrated with HCl (0.5N) using phenolphthalein as indicator.

2.3Microflotation tests

Microflotation tests were carried out at room temperature (around 25°C) in a modified Hallimond tube (addition of an extender between the bottom and the upper part of the tube, in order to reduce the effects of hydraulic entrainment) with 320mL of internal volume. The airflow was kept at 40cm3/min and the air pressure at 10psi in order to minimize the hydraulic entrainment. The mineral mass used in each test was 1.0g with particle size between +106–150μm (+150–100 #). The conditioning time was 7min and the flotation time was 1min.

A first experimental campaign was carried out in order to study the influence of the saponified BYC aging on the apatite recovery, at pH 9 and biocollector dosage of 400mg/L. Both flotation test and SI measurement were carried out at the same time. With the results of the first experimental campaign, it was established an optimal aging period for the BYC.

Five different dosages of aged saponified BYC (50, 200, 400, 600, and 800mg/L) and four different pH values (7, 8, 9, and 10) were tested. The alkaline pH was chosen to match the pH industrially adopted in Brazil for phosphate rock flotation (normally around 10–10.5). The apatite flotation in neutral pH (7) was also tested, which could lead to a cost reduction with pH regulators. BYC has a theoretical lipid mass composition of 12.6% (db) [15]. Therefore, the tested dosage range is similar to dosages of fatty acids collectors tested by other authors in apatite flotation [7,18,19]. Flotigam 5806 from Clariant, a synthetic mix of fatty acids highly used in phosphate rock flotation worldwide, was used as a collector benchmark and it was prepared following the manufacturer recommendations. Three different pHs (8, 9, and 10) and four dosages (2.5, 5.0, 7.5, and 10.0mg/L) were tested. The neutral pH was not tested because Clariant suggests that Flotigam 5806 must be used at a pH range from 8 to 10, with dosages between 50 and 300g/ton. The slurry pH was adjusted with HCl and NaOH, both at 1%. Distilled water was used throughout the experiments. All tests were performed in triplicate.

3Results and discussion

Table 1 shows the XRF results of the apatite samples. Regarding the chemical composition, it is possible to assume that apatite samples had around 95.5% of purity, with presence of traces of Cl, indicating a possible presence of fluorapatite.

Table 1.

XRF results for the apatite samples (in %).

CaO  P2O5  K2SiO2  SO3  Cl  Na2ThO2  Fe2O3  MnO  BaO  SrO 
54.02  38.49  4.20  1.10  0.63  0.39  0.34  0.27  0.18  0.11  0.11  0.10  0.06 

Fig. 1 shows the SEM images (BEC and SEI) of the apatite sample. It is possible to notice the association of apatite with a secondary acicular mineral phase. EDS results are shown for two points, one on the acicular phase (Fig. 1c) and the other on the apatite phase (Fig. 1d). Fig. 1d shows that the main mineral phase is composed by Ca, P, and O, as expected for apatite (Ca5(PO4)3). Since Cl and F were also observed, this could be an indication of the existence of fluorapatite and chlorine-apatite in the sample (Ca5(PO4)3(F,Cl)). The existence of the fluorine–chlorine–apatite phase agrees with the XRF results and does not affect its flotation in anyway. The acicular phase (Fig. 1c) could not be correctly identified based only on this result. The presence of Ag in the EDS spectra of the secondary mineral phase is rather unusual considering its association with igneous apatite.

Figure 1.

SEM images for apatite sample: (a) BEC and (B) SEI image at ×750 magnification. EDS results for points (c) 1 and (d) 2 marked in (a).


Fig. 2 shows the XRD diffraction pattern for the apatite sample. A peak match correlation of 95.75% was obtained between the sample and fluorapatite reference diffraction pattern 96-901-3556. A semi-quantitative analysis using the Reference Intensity Ratio method showed that the sample was composed by 100% of fluorapatite. When the apatite sample XRD pattern was compared with chlorine-apatite (references 96-901-3555 and 96-901-3557), the peak match correlation was only 23.06%, confirming that the sample was mainly composed by fluorapatite.

Figure 2.

Apatite sample X-ray diffraction.


Regarding the Zeta potential measurements (Fig. 3a), the apatite isoelectric point (IEP) was not detected in the tested pH range (3.5–12.5) and the apatite surface charge remained negative in all measurements. According to the literature, apatite has negative superficial charge in a solution with alkaline pH, which agrees with the results found, and the adsorption of anionic collectors on its surface is due to chemisorption [20]. Fig. 3b presents the granulometric analyses of the apatite sample. It is possible to notice the presence of particles below 103μm (68.3%) and above 175μm (20%), even though the samples had been size classified through wet sieved (+106–150μm). This fact can be explained by the imperfection of the sieving process and by possible mistakes in the laser diffraction analysis related to the particle morphology.

Figure 3.

(a) Zeta potential and (b) particle size distribution for the apatite sample.


Fig. 4 shows the influence of the saponified BYC aging on both collector's SI and average apatite recovery. During the aging process, no visual or aromatic changes in the collector was detected. The BYC SI increased abruptly in the first 24h after the saponification and did not stabilize during the testes, even after 144h. On the other hand, the apatite recovery stabilized around 94% when using saponified BYC with aging ≥96h (selected as the optimal aging). The results indicate that even though saponification reactions still occur after 96h, apparently they do not have major influence on apatite recovery. For the 96h-aged solution, the average apatite recovery was 94.9±0.4% and the SI was 76.7±1.4mg KOH/g. The found SI was lower than the observed for fruit's oil used as fatty acids sources in apatite flotation, such as 192±3.5mg KOH/g for pequi [18] and values ranging from 196 to 212mg KOH/g for Amazon fruits [19], but the apatite recovery was as high as found by the same authors. It is possible to notice that apatite recoveries were considerably low in the first for 24h-aged saponified BYC (around 35%), indicating that freshly prepared saponified BYC should not be used in flotation. A major increase in the apatite recovery was noticed after 48h of aging when compared with the results for 24h (2.19 times higher). A linear correlation between BYC SI and the apatite recovery was obtained with a Pearson correlation coefficient of 93% (which is inside the 95% confidence interval).

Figure 4.

Influence of the saponified BYC aging on the collector SI and on the average apatite recovery.


Apatite flotation test results using the 96h-aged saponified BYC and the Flotigam 5806 are presented in the Fig. 5. Since the error bars for the biocollector presented in the figure are too small, the complete data set (average apatite recovery and standard deviation) for the 96h-aged saponified BYC test is presented in Table 2. A non-linear fitting (exponential decay) was used in order to fit the experimental data and to model the recovery stabilization plateau. The occurrence of such plateau is a regular feature in the flotation of many minerals. In sphalerite flotation using Bacillus circulans as collector, for example, the plateau was found for biocollector dosages above 2mg/g [12].

Figure 5.

Apatite flotation results at pH (a) 7, (b) 8, (c) 9, and (d) 10 using 96h-aged saponified BYC and Flotigam 5806.

Table 2.

Apatite flotation data set using 96h-aged saponified BYC.

Dosage (mg/L)  pH 7pH 8pH 9pH 10
  Avg. Re. (%)  Std. Dev. (%)  Avg. Re. (%)  Std. Dev. (%)  Avg. Re. (%)  Std. Dev. (%)  Avg. Re. (%)  Std. Dev. (%) 
0.00  0.00  0.00  0.00  0.00  0.00  0.00  0.00 
50  48.41  0.19  47.26  0.90  31.41  0.52  8.36  0.23 
200  97.39  0.16  97.36  0.27  96.60  0.53  95.96  0.16 
400  97.15  0.64  97.35  0.28  97.20  0.29  95.53  0.25 
600  96.81  0.32  97.38  0.16  97.37  0.62  94.93  0.39 
800  95.96  0.42  97.82  0.17  96.55  0.44  95.45  0.27 

Average apatite recoveries above 95% were obtained for the 96h-aged saponified BYC at dosages ≥200mg/L in all tested pHs, including pH 7. This is a remarkable result, since fatty acids, the main industrially adopted collector for apatite in Brazil, usually do not show high recoveries at this pH. An industrial flotation of phosphate rock performed at pH 7 could lead to cost reduction with pH regulators and to the reduction of the electrolyte load in the slurry (especially regarding Na+). The pH influence on the apatite recovery with the 96h-aged saponified BYC was evident for the dosage of 50mg/L. The pH increases from 7 to 10 promoted a reduction in the average apatite recovery with the biocollector from 48.4±0.2 to 8.4±0.2, respectively.

For the Flotigam 5806, the best results were obtained for dosages ≥5.0mg/L. The exception was at pH 10, in which a high average apatite recovery was also obtained for the dosage of 2.5mg/L. Lower dosages of Flotigam 5806 were required to produce similar apatite recovery when compared to the 96h-aged saponified BYC. However, high apatite recoveries were obtained with the 96h-aged saponified BYC in all tested pHs, except on pH 10, where the results for the two collectors were similar.

The pH influence on Flotigam 5806 was also verified. An increase in the pH produced an increase in the apatite recovery when using Flotigam 5806, which agrees with industrial data and explains the adoption of pH around 10 in the phosphate rock flotation. On the other hand, for the 96h-aged saponified BYC an increase in the pH produced a decrease in the apatite recovery.

The high apatite recovery could also be related with other constituents of the BYC. S. cerevisiae potential for carboxylic acid production had been suggested in the literature [21] and the interaction between some of the carboxylic acid's functional groups present in the SC and the apatite surface cannot be neglected. Proteins and nucleic acids were present in the saponified BYC solution since no cellular constituent separation was performed, and the adopted preparation and saponification methodology possibly exposed the cell's DNA, which has reported biocollector properties [12].

Saponified fatty acids are well known as frother and ionized species can generate excessively stable froths [8]. No major differences were visually found between the 96h-aged saponified BYC and the Flotigam 5806 regarding the froth aspect and stability, suggesting that the biocollector also acted as a frother.


A first attempt to use saponified BYC (S. cerevisiae) as a biocollector in apatite flotation was carried out. High-purity apatite crystals were prepared and characterized. The influence of the biocollector aging in the apatite recovery was investigated and a linear correlation, with statistical significance, was found. The optimal aging of 96h was established. Average apatite recoveries above 95% were obtained with dosages ≥200mg/L of 96h-aged saponified BYC. The average apatite recovery was not affected by changes in the pH for the biocollector at this dosage (no statistically significant difference was found). As expected, the average recoveries with the industrially adopted collector (Flotigam 5806) were around 93%. However, higher apatite recoveries were obtained with 96h-aged saponified BYC in almost all tests carried out. Regarding the froth, no major differences were visually found between the two tested collectors.

An inverse behavior regarding the pH change was observed between the Flotigam 5806 and the biocollector. An increase in the pH produced and the increase in the apatite recovery when using the Flotigam 5806 and a decrease when using the 96h-aged saponified BYC. The pH reduction in the industrial flotation of apatite (from 10 to 7) could be an important step forward for the mineral industry. The reduction in the use of pH modifiers directly reduces operational costs, the electrolyte load in the slurry, and could also have beneficial effects on the recirculating water quality.

More tests with phosphate rock are required to ensure the potential use of saponified BYC as an industrial biocollector. Proteins and nucleic acids were present in the saponified BYC solution and the interaction of these constituents with the apatite surface is not clear so far. Therefore, more studies are required to understand the adsorption mechanisms.

5Conflicts of interest

The authors declare no conflicts of interest.


The authors gratefully acknowledge the financial support of the Brazilian agencies CNPq, CAPES, FAPEG, and FUNAPE. In addition, the authors would like to thank Copebras, Labmic, UNIFESSPA, UFOP, UFG, and TU Clausthal.

S. Murphy, D. Burch, J. Clapp.
Cereal Secrets: The World's Largest Grain Traders and Global Agriculture.
Oxfam Research Reports, (2012),
B.F.T. Rudorff, D.A. Aguiar, W.F. Silva, L.M. Sugawara, M. Adami, M.A. Moreira.
Studies on the rapid expansion of sugarcane for ethanol production in São Paulo state (Brazil) using Landsat data.
Remote Sensing, 2 (2010), pp. 1057-1076
L.I. Prochnow.
Optimizing nutrient use in low fertility soils of the tropics.
Better Crops, 92 (2008), pp. 19-21
A.S.R. Juo, K. Franzluebbers.
Tropical Soils: Properties and Management for Sustainable Agriculture.
1st ed, Oxford University Press, (2003),
Current World Fertilizer Trends and Outlook to 2016. Rome: Food and Agriculture Organization of the United Nations; 2012.
H. Sis, S. Chander.
Reagents used in the flotation of phosphate ores: a critical review.
Min. Eng., 16 (2003), pp. 577-585
A.C. Silva, I.L.A. Moraes, E.M.S. Silva, C.M. Silva Filho.
Jatropha curcas L. oil selectivity in froth flotation.
Int. J. Biol. Biomol. Agric. Food Biotechnol. Eng., 10 (2016), pp. 1-7
R.C. Guiamarães, A.C. Araújo, A.E.C. Peres.
Reagents in igneous phosphate ores flotation.
Minerals Eng., 18 (2004), pp. 199-204
S.K. Behera, A.F. Mulaba-Bafubiandi.
Microbes assisted mineral flotation a future prospective for mineral processing industries: a review.
Mineral Process. Extractive Metall., 38 (2016), pp. 1-10
A.G. Merma, M.L. Torem, J.J.V. Morán, M.B.M. Monte.
On the fundamental aspects of apatite and quartz flotation using a Gram positive strain as a bioreagent.
Min. Eng., 48 (2013), pp. 61-67
L.Y. Lopez, A.G. Merma, M.L. Torem, G.H. Pino.
Fundamental aspects of hematite flotation using the bacterial strain Rhodococcus ruber as bioreagent.
Min. Eng., 75 (2015), pp. 63-69
B. Vasanthakumar, H. Ravishankar, S. Subramanian.
Microbially induced selective flotation of sphalerite from galena using mineral-adapted strains of Bacillus megaterium.
Colloids Surf. B, 112 (2013), pp. 279-286
K.H. Rao, S. Subramanian.
Bioflotation and bioflocculation of relevance to minerals bioprocessing.
Microbial Processing of Metal Sulfides, pp. 267-286
R. Dwyer, W.J. Bruckard, S. Rea, R.J. Holmes.
Bioflotation and bioflocculation review: microorganisms relevant for mineral beneficiation.
Min. Process. Extractive Metall., 121 (2012), pp. 65-71
C. Borchani, F. Fonteyn, G. Jamin, M. Paquot, C. Blecker, P. Thonart.
Enzymatic process for the fractionation of baker's yeast cell wall (Saccharomyces cerevisiae).
Food Chem., 163 (2014), pp. 108-113
S.U. Padukone, K.A. Natarajan.
Microbially induced separation of quartz from calcite using Saccharomyces cerevisiae.
Colloids Surf. B, 88 (2011), pp. 45-50
D.L. Badiu, A.M. Balu, L. Barbes, R. Luque, R. Nita, M. Radu, E. Tanase, N. Rosoiu.
Physico-chemical characterisation of lipids from Mytilus galloprovincialis (L.) and Rapana venosa and their healing properties on skin burns.
Lipids, 43 (2008), pp. 829-841
A.C. Silva, E.M.S. Silva, T.C. Silva, B.E. Alves.
Apatite microflotation using pequi oil.
Min. Process. Extractive Metall., 124 (2015), pp. 233-239
Costa, D., 2012. Uso de óleos vegetais amazônicos na flotação de minérios fosfáticos. PhD thesis, School of Engineering, Federal University of Minas Gerais, Belo Horizonte, Brazil.
M.S. Oliveira.
Minério fosfático sílico-carbonatado: estudo fundamental [doctor thesis].
Universidade Federal de Minas Gerais, (2007),
A.G. Sandström, H. Almqvist, D. Portugal-Nunes, D. Neves, G. Lidén, M.F. Gorwa-Grauslund.
Saccharomyces cerevisiae: a potential host for carboxylic acid production from lignocellulosic feedstock?.
Appl. Microbiol. Biotechnol., 98 (2014), pp. 7299-7318
Copyright © 2018. Brazilian Metallurgical, Materials and Mining Association
Journal of Materials Research and Technology

Subscribe to our newsletter

Article options
Cookies policy
To improve our services and products, we use cookies (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here.